Journal of Environmental Pathology, Toxicology and Oncology

ISSN for PRINT: 0731-8898

For Online Access

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2002, Volume21

Issue 1

98 pages

Issue price - $160.00

Slawomir Terlikowski
Department of Gynaecology and Septic Obstetrics, Medical Academy of Bialystok, Bialystok, Poland

Mariola Sulkowska
Department of Pathological Anatomy, Medical Academy of Bialystok, Bialystok, Poland

Henryk F. Nowak
Department of Pathological Anatomy, Medical Academy of Bialystok, ul. Waszyngtona 13, 15-269 Bialystok 8, Poland

We studied the antitumor effect of recombinant human tumor necrosis factor-a (rh TNF-a) on the intraperitoneal (i.p.) growth of Ehrlich ascites tumor (EAT) in Swiss albino male mice. The animals were treated with i.p. injection of rh TNF-a in doses of 5, 7.5, or 10 mg three times a week for 2 weeks, respectively, starting on the 4th day after the EAT inoculation. The effect of the cytokine was evaluated based on the following parameters: total ascites volume, packed cell volume, total packed cell volume, inhibitory growth rate, cellular population of EAT, morphological EAT cell changes, and mean survival time (MST). Rh TNF-a in a dose of 5 amg had only a slight effect on MST and inhibitory growth rate (IGR). After a dose of 7.5 mg, an increased IGR (p < 0.01) was observed, but the animals did not live longer than the controls. After 7.5- and 10-mg doses (p < 0.001), the number of cells in EAT decreased significantly and enhanced cellular damage to EAT cells was found. In mice treated with 10 mg, a significant IGR (p < 0.001) was accompanied by enhancement of MST (p < 0.01). Although the 10 mg dose exerted a greater effect compared with the remaining doses, no complete regression was attained.

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