Journal of Environmental Pathology, Toxicology and Oncology

ISSN for PRINT: 0731-8898

For Online Access

Best Paper Award Selection - Editorial Board Site

2002, Volume21

Issue 1

98 pages

Issue price - $160.00

Qingyang Gu
Department of Pathology, Beijing Institute of Radiation Medicine, Beijing, China

Dewen Wang
Department of Pathology, Beijing Institute of Radiation Medicine, Beijing, China

Yabing Gao
Department of Pathology, Beijing Institute of Radiation Medicine, Beijing, China

Jie Zhou
Institute of Microbiology and Epidemiology, Academy or Military Medical Sciences, Beijing, China

Ruiyun Peng
Department of Pathology, Beijing Institute of Radiation Medicine, Beijing, China

Yufang Cui
Department of Pathology, Beijing Institute of Radiation Medicine, Beijing, China

Guowei Xia
Department of Pathology, Beijing Institute of Radiation Medicine, Beijing, China

Quanhong Qing
Department of Pathology, Beijing Institute of Radiation Medicine, Beijing, China

Hong Yang
Department of Pathology, Beijing Institute of Radiation Medicine, Beijing, China

Jie Liu
Department of Pathology, Beijing Institute of Radiation Medicine, Beijing, China

Meilan Zhao
Department of Pathology, Beijing Institute of Radiation Medicine, Beijing, China

Radiation-impaired wound is characterized by delayed healing, nonhealing, and carcinogenesis. The mechanism remains unclear. Matrix metalloproteinases (MMPs) are one family of key regulators of the process of wound healing. Their abnormal expression plays important roles in the formation of some chronic skin ulcers. The objective of this project was to study the expression of MMP1 in surgical and radiation-impaired wound healing and its effects on the healing process and tissue remodeling. A rat model of radiation-impaired wound healing was used. Routine light microscopy, electron microscopy, immunohistochemistry, and in situ hybridization, all of which enabled the detection of MMPI expression during the healing process, were performed. The wound healing process was impaired and delayed. In rats receiving 25Gy g-ray locally, the irradiated wounds healed 6 days later than the nonirradiated controls. The following changes in MMP1 expression were found: (1) In the early inflammatory phase and in the period of granulation tissue formation, MMP1 expression was only slightly if at all affected in the newly formed epidermis of irradiated wounds compared with controls. Later, the epidermal expression of MMP1 in radiation wounds was comparatively increased following the delay of the healing process. (2) MMP1 expression in irradiated wounds was markedly decreased in fibroblasts, endothelial cells, and macrophages compared with controls. The expression phase was prolonged because of the delay of the healing process. The reduced expression of MMP1 in granulation tissue retards such important processes as cell migration, angiogenesis, and tissue remodeling, thus slowing the healing process. The expression ofMMPI in the proliferating keratinocytes may help re-epithelialization. However, in the late healing period, overexpression ofMMPI in the epidermis may hinder the establishment ofbasal membrane and the formation of granulation tissue, and affect the tissue remodeling process.

Download article, 8 pages

Article price - $35.00

<< Previous article   Next article >>