ITEMS

modern scholarly publishers in the finest tradition

Journals

	Search

Journal of Environmental Pathology, Toxicology and Oncology

ISSN for PRINT: 0731-8898

Institutional price:	$672.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2002, Volume21

98 pages

Issue price - $160.00

Expression of Telomerase Reverse Transcriptase in Radiation-Induced Chronic Human Skin Ulcer

Po Zhao
Department of Pathology, Chinese Department of Pathology, Chinese General Hospital of People's Liberation Army, 28 Fuxing Road, 100853, Beijing, China

Li Zhijun
Department of Pathology, Chinese General Hospital of People's Liberation Army, Beijing, China

Mei Zhong
Department of Pathology, Chinese General Hospital of People's Liberation Army, Beijing, China

Qingyang Gu
Department of Pathology, Beijing Institute of Radiation Medicine, Beijing, China

Dewen Wang
Department of Pathology, Beijing Institute of Radiation Medicine, Beijing, China

ABSTRACT

The objective of this study was to investigate the expression of the catalytic subunit of telomerase, telomerase reverse transcriptase (TRT), and the possible relationship between the TRT expression and poor healing or cancer transformation in radiation-induced chronic human skin ulcer. Rabbit antibody to human TRT and SP immunohistochemical method were used to detect TRT expression in 24 cases of formalin-fixed, paraffin-embedded chronic human skin ulcer tissues induced by radiation, 5 cases of normal skin, 2 of burned skin, and 8 of cancer. The positive rate of TRT expression in chronic radiation ulcers was 58.3% (14/24), of which it was strongly positive in 41.7% cases (10/24) and weakly positive in 16.7% (4/24). TRT expression was 0% in normal (0/5) and burned skin (0/2), and 100% in cancer cases (8/8). The strongly positive expression of TRT was observed almost always in the cytoplasm and nucleus of squamous epithelial cells of the epidermis but it was negative or only weakly positive in the smooth muscle and endothelia of small blood vessels and capillaries, and in fibroblasts. Chronic inflammatory cells, plasmacytes, and lymphocytes were weakly positive for TRT. TRT expression could be involved in the poor healing caused by sclerosis of small blood vessels and lack of granulation tissue and in the cancer transformation of chronic radiation ulcer.