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Journal of Environmental Pathology, Toxicology and Oncology

ISSN for PRINT: 0731-8898

Institutional price:	$672.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2001, Volume20

98 pages

Issue price - $160.00

DNA Repair Studies: Experimental Evidence in Support of Chicken DT40 Cell Line as a Unique Model

Pawan Kumar Dhar
Department of Radiation Genetics, Faculty of Medicine, Kyoto University, Kyoto, Japan

Eiichiro Sonoda
Department of Radiation Genetics, Faculty of Medicine, Kyoto University, Kyoto, Japan

Akira Fujimori
Department of Radiation Genetics, Faculty of Medicine, Kyoto University, Kyoto, Japan

Yukiko M. Yamashita
Department of Radiation Genetics, Faculty of Medicine, Kyoto University, Kyoto, Japan

Shunichi Takeda
Department of Radiation Genetics, Faculty of Medicine, Kyoto University, Kyoto, Japan

ABSTRACT

DT40 is a chicken B lymphocyte cell line that exhibits a high ratio of targeted and random integration of transfected DNA constructs. Using the DT40 cell line makes it comparatively easy to disrupt multiple genes in a single cell and to generate conditional targeted mutants including tet-controlled cre-lox-mediated and temperature-sensitive mutants. The DT40 mutants show a strong phenotypic resemblance to murine mutants with respect to genes involved in DNA recombination and repair. Because of these characteristics, DT40 is an attractive model for the analysis of DNA recombination and repair studies in vertebrates despite obvious concerns associated with the use of a transformed cell line that may have certain cell-line-specific characteristics. We present experimental evidence to demonstrate the usefulness of the DT40 cell line as a unique model to study DNA damaging events and their associated repair pathways.