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Journal of Environmental Pathology, Toxicology and Oncology

ISSN for PRINT: 0731-8898

Institutional price:	$672.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2006, Volume25

94 pages

Issue price - $172.00

Electroporation Enhances Radiation and Doxorubicin-Induced Toxicity in Solid Tumor In Vivo

Pratip Shil
Biophysics Laboratory, Department of Physics, University of Pune, Pune 411007 India

Amit Kumar
Radiation Biology & Health Sciences Division, Bhabha Atomic Research Center, Mumbai 400095, India

Pandit B. Vidyasagar
Biophysics Laboratory, Department of Physics, University of Pune, Pune 411007 India

Kaushala Prasad Mishra
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Center, Mumbai 400095,India. Present address: Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8553, Japan

ABSTRACT

Treatment of cancer patients is subject to limitations in radiotherapy and chemotherapy. This necessitates development of new protocols, and the present work reports on the effects of a combination of local electroporation with ionizing radiation and/or anticancer drug doxorubicin hydrochloride (DOX) on subcutaneous solid tumor murine fibrosarcoma. Localized treatment of fibrosarcoma tumor, grown in right hind leg of Swiss mice, has been carried out using DOX (0.6 mg/kg body weight), radiation (Co 60 γ-rays, dose rate 0.37 Gy/min) and electroporation (1 kV/cm, 200 μs, 8 pulses per burst, 10 bursts) individually or in combinations. Measurements of the tumor growth kinetics after treatment with combinations have revealed significant growth delay. The treatment groups, (i) radiation and electroporation, (ii) DOX and electroporation, and (iii) radiation, DOX and electroporation, have yielded tumor growth delays (TGDs) of 1.22, 1.5, and 1.73 days, respectively, compared to control with the tumor volumes being 53%, 57%, and 49% that of control on the final day of observation. These results suggest that the antitumor effects of a moderate dose of γ radiation and low concentration of DOX can be significantly enhanced by combination with electroporation.