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Journal of Environmental Pathology, Toxicology and Oncology

ISSN for PRINT: 0731-8898

Institutional price:	$672.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2007, Volume26

89 pages

Issue price - $187.00

Radioprotection against DNA Damage by an Extract of Indian Green mussel, Perna viridis (L)

Sreekumar P. Kumaran
National Institute of Oceanography, Dona Paula, Goa-403 004, India

Binoj C. Kutty
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400 085, India

Anil Chatterji
National Institute of Oceanography, Dona Paula, Goa-403 004, India

Parameswaran P. Subrayan
National Institute of Oceanography, Dona Paula, Goa-403 004, India

Kaushala Prasad Mishra
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Center, Mumbai 400095,India. Present address: Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8553, Japan

ABSTRACT

This study describes the radioprotective ability of a hydrolysate prepared using an enzymeacid hydrolysis method from the green mussel Perna viridis in terms of its ability to prevent radiation-induced damage in plasmid DNA, cell death, reactive oxygen species (ROS) formation, and DNA damage in mice lymphocytes. The mussel hydrolysate (MH) present during irradiation showed significant protection from γ-radiation-induced strand breaks in plasmid DNA as evaluated by gel electrophoresis. Viability studies by trypan blue dye exclusion and MTT assay showed that preincubation of mice splenic lymphocytes with MH protected them from γ-radiation-mediated killing. Moreover, the presence of MH during irradiation of isolated mice lymphocytes significantly decreased the DNA damage, as measured by comet assay. Measurement of intracellular ROS by dichlorofluorescein fluorescence revealed that the presence of MH effectively reduced the ROS generated in lymphocytes by both chemical method and γ-irradiation. Prevention of DNA damage both in plasmid and lymphocytes and cell death in lymphocytes appears correlated with reduction of oxidatively generated free radicals. It is concluded that protection against radiation-induced cell death and DNA damage by MH was attributable to reduction of reactive free radical species generated by γ-radiation.