Journal of Environmental Pathology, Toxicology and Oncology

ISSN for PRINT: 0731-8898

For Online Access

Best Paper Award Selection - Editorial Board Site

2006, Volume25

Issue 1-2

546 pages

Issue price - $344.00

Henry Hirschberg
Department of Health Physics, University of Nevada, Las Vegas, 4505 Maryland Pkwy. Box 453037, Las Vegas, NV 89154-3037; and Beckman Laser Institute, University of California, 1002 Health Science Road, East Irvine, CA 92612, USA

Dag R. Sorensen
Center for Comparative Medicine, Rikshospitalet, Oslo, Norway

Even Angell-Petersen
Department of Surgical Oncology, The Norwegian Radium Hospital, Oslo, Norway

Qian Peng, PhD
Pathology Clinic, Rikshospitalet-Radiumhospitalet HF Medical Center, Faculty Division Radiumhospitalet, University of Oslo, Montebello, N-0310 Oslo, Norway

Bruce Tromberg
Beckman Laser Institute, University of California, 1002 Health Science Road, East Irvine, CA 92612

Chung-Ho Sun
Beckman Laser Institute, University of California, 1002 Health Science Road, East Irvine, CA 92612

Signe Spetalen
Department of Pathology, Ullevaal University Hospital, Oslo, Norway

Steen J. Madsen
Department of Health Physics, University of Nevada Las Vegas, 4505 Maryland Parkway, Las Vegas, NV 89154-3037, and UNLV Center for Molecular Medicine and Radiation Biology, 4505 Maryland Parkway, Las Vegas, NV 89154, USA

The probability of achieving local control with current single-shot, intraoperative photodynamic therapy (PDT) treatments of intracerebral gliomas seems improbable due to the length of time required to deliver adequate light fluences to depths of 1−2 cm in the resection margin. Additionally, due to the short doubling time of many malignant gliomas, the kill rate per cell doubling indicates that it seems unlikely that a single treatment would be sufficient to prevent tumor recurrence. Multiple repetitive treatments would therefore seem required. In this publication we primarily review our work examining the effects of repetitive PDT on malignant brain tumor cells both in vitro and in vivo. The in vitro therapy response of human and rat glioma spheroids to 5-aminolevulinic acid (ALA)-mediated PDT in repetitive form was investigated. The results indicated that PDT repeated at relatively long intervals (weeks) was more effective at inhibiting spheroid growth than either daily fractionated PDT or single-treatment regimes. The in vivo response to repetitive treatment was evaluated in a rodent glioma model where BT4C cell line tumors were established in the brains of inbred BD-IX rats. Microfluorometry of frozen tissue sections showed that PpIX is produced with a 10−20:1 tumor to normal tissue selectivity ratio 4 hr after ALA injection. Preliminary evidence of increased efficacy of repetitive PDT and low fluence rate treatment is presented.

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