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Journal of Environmental Pathology, Toxicology and Oncology

ISSN for PRINT: 0731-8898

Institutional price:	$672.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2003, Volume22

82 pages

DOI: 10.1615/JEnvPathToxOncol.v22.i1

Issue price - $160.00

Curcumin Exhibits Antimetastatic Properties by Modulating Integrin Receptors, Collagenase Activity, and Expression of Nm23 and E-Cadherin

Amitava Chatterjee
Department of Receptor Biology and Tumor Metastasis, Chittaranjan National Cancer Institute, 37 S.P. Mukherjee Road, Kolkata-700 026, India

Aparna Mitra
Department of Receptor Biology and Tumor Metastasis, Chittaranjan National Cancer Institute, 37 S.P. Mukherjee Road, Kolkata-700 026, India

Subrata Ray
Department of Receptor Biology and Tumor Metastasis, Chittaranjan National Cancer Institute, Calcutta, India

Nibedita Chattopadhyay
Department of Receptor Biology and Tumor Metastasis, Chittaranjan National Cancer Institute, Calcutta, India

Maqsood Siddiqi
Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute; and Bose Institute, Calcutta, India

ABSTRACT

Curcumin (diferuloyl methane), the major pigment from the rhizome of Curcuma longa L., has been widely studied for its tumor-inhibiting properties. Recent studies indicate that curcumin can modify cell receptor binding; it also affects intracellular signaling reactions. Curcumin-treated B16F10 melanoma cells formed eight-fold fewer lung metastases in C57BL6 mice. In the cell adhesion assays, curcumin-treated cells showed a dose-dependent reduction in their binding to four extracellular matrix (ECM) proteins. The binding to fibronectin, vitronectin, and collagen IV decreased by over 50% in 24 hours, and by 100% after 48 hours of curcumin treatment; it persisted at this level even after 15 days of cultivating cells in curcumin-free medium. Curcumin-treated cells showed a marked reduction in the expression of a5b1 and avb3 integrin receptors. In addition, curcumin treatment inhibited pp125 focal adhesion kinase (FAK), tyrosine phosphorylation of a 120 kD protein, and collagenase activity. Curcumin enhances the expression of antimetastatic proteins, tissue inhibitor metalloproteinase (TIMP)-2, nonmetastatic gene 23 (Nm23), and E-cadherin. In this article we report on the effect of curcumin on the expression of integrin, TIMP-2, Nm23, E-cadherin, adhesion, and metalloproteinase activity.