Critical Reviews™ in Eukaryotic Gene Expression

ISSN for PRINT: 1045-4403

For Online Access

Best Paper Award Selection - Editorial Board Site

2003, Volume13

Issue 2-4

227 pages

DOI: 10.1615/CritRevEukaryotGeneExpr.v13.i24

Issue price - $507.00

Anne L. Symons
School of Dentistry, University of Queensland, 200 Turbot Street, Brisbane Qld 4000, Australia

Arosha Weerakoon
School of Dentistry, University of Queensland, 200 Turbot Street, Brisbane Qld 4000, Australia

Sandy C. Marks, Jr.
Department of Cell Biology, University of Massachusetts Medical Center, Worcester 01655, USA

In the toothless (tl/tl) osteopetrotic rat, teeth form but fail to erupt. Treatment of tl/tl rats with colony-stimulating factor-1 (CSF-1) activates bone resorption by osteoclasts, permits tooth eruption, and upregulates the immunoreactivity of bone marrow mononuclear cells to growth hormone receptor (GHr) and insulinlike growth factor (IGF)-I. This study examined the distribution of tartrate-resistant acid phosphatase (TRAP) and immunoreactivity for GHr and IGF-I in osteoclast-like cells located on the alveolar bone margin, adjacent to the lower first molar crown, in 14-day-old normal and tl/tl rats, following treatment with CSF-1. Osteoclast-like cells demonstrated a positive reaction for TRAP, GHr, and IGF-I in all groups. However, in tl/tl tissue, osteoclast-like cells were generally negative for GHr. There was no significant difference in the total number of TRAP-, GHr-, and IGF-I-positive osteoclast-like cells on the adjacent bone margin in normal, normal treated with CSF-1, and tl/tl rats. CSF-1 treatment of the tl/tl rat significantly increased the total number of osteoclast-like cells, which were positive for TRAP (p < 0.001), GHr (p < 0.05) and IGF-I (p < 0.01).

DOI: 10.1615/CritRevEukaryotGeneExpr.v13.i24.120 Download article, 10 pages

Article price - $35.00

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