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Critical Reviews™ in Eukaryotic Gene Expression

Published 6 issues per year

ISSN Print: 1045-4403

ISSN Online: 2162-6502

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.6 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 2.2 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00058 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.33 SJR: 0.345 SNIP: 0.46 CiteScore™:: 2.5 H-Index: 67

Indexed in

SCE Jumping: Genetic Tool for Allelic Exchange in Bacteria

Volume 14, Issue 1&2, 2004, 12 pages
DOI: 10.1615/CritRevEukaryotGeneExpr.v14.i12.30
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ABSTRACT

As more microbial genome sequence information becomes available, the field of bacterial patho-genesis would benefit from the development of new genetic tools designed to facilitate gene function studies on a genomic scale. The complete DNA sequence of the bacterium Pseudomonas aeruginosa provides an opportunity to apply functional genomics to a major opportunistic human pathogen. Here, I describe the development of a new gene replacement scheme termed "SCE jumping" in P. aeruginosa. The system uses the yeast I-SceI homing endonuclease in conjunction with in vitro mariner-transposon mutagenesis to generate mutations within targeted regions of the chromosome for genetic footprinting. Use of SCE jumping for generating transposon insertion mutants is anticipated to be widely applicable to other bacterial organisms. This allelic exchange strategy is discussed in context with other methods of gene replacement strategies available in P. aeruginosa. Development of SCE jumping provides an excellent example of the power of importing systems from unrelated organisms to circumvent practical challenges in molecular genetics.

CITED BY
  1. Kalivoda Eric J., Horzempa Joseph, Stella Nicholas A., Sadaf Aroba, Kowalski Regis P., Nau Gerard J., Shanks Robert M. Q., New Vector Tools with a Hygromycin Resistance Marker for Use with Opportunistic Pathogens, Molecular Biotechnology, 48, 1, 2011. Crossref

  2. Hafez Mohamed, Hausner Georg, Bonen L., Homing endonucleases: DNA scissors on a mission, Genome, 55, 8, 2012. Crossref

  3. Wong Sandy M.S., Akerley Brian J., Identification and Analysis of Essential Genes in Haemophilus influenzae, in Microbial Gene Essentiality: Protocols and Bioinformatics, 416, 2008. Crossref

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