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International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

Institutional price:	$538.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2004, Volume6

118 pages

DOI: 10.1615/IntJMedMushr.v6.i4

Issue price - $128.00

Antitumor and Immunomodulatory Effects of Culinary-Medicinal Shiitake Mushroom Lentinus edodes (Berk.) Singer: Analysis of NK Activity, Lymphoproliferative Response, and Antibody Production

Ramon Kaneno
Department of Microbiology and Immunology, Institute of Bioscience of Botucatu/UNESP, P.O. Box 510, ZC 18618-000, Botucatu, SP, Brazil

Andrea V. F. Belik
Department of Microbiology and Immunology, Institute of Bioscience of Botucatu/UNESP, Botucatu, SP, Brazil

Fabiani G. Frantz
Department of Microbiology and Immunology, Institute of Bioscience of Botucatu/UNESP, Botucatu, SP, Brazil

Juliana S. F. Lorenzo
Department of Microbiology and Immunology, Institute of Bioscience of Botucatu/UNESP, Botucatu, SP, Brazil

Luciana M. Fontanari
Department of Microbiology and Immunology, Institute of Bioscience of Botucatu/UNESP, Botucatu, SP, Brazil

Lindsey Castoldi
Department of Microbiology and Immunology, Institute of Bioscience of Botucatu/UNESP, Botucatu, SP, Brazil

Bianca R. Dias
Department of Microbiology and Immunology, Institute of Bioscience of Botucatu/UNESP, Botucatu, SP, Brazil

Ana L. T. Spinardi Barbisan
Department of Pathology, Faculty of Medicine of Botucatu/UNESP, Botucatu, SP, Brazil

Augusto Ferreira da Eira
Department of Plant Production, Agriculture Microbiology, FCA/UNESP, Botucatu, SP, Brazil

ABSTRACT

We evaluated the efficiency of 12 aqueous extracts obtained at three different temperatures from four strains of culinary−medicinal Shiitake mushroom Lentinus edodes in protecting mice against the development of Ehrlich carcinoma. We observed first that the strains Le 96/17 and Le 95/ 01 of L. edodes were more efficient than JAB/k and Le 96/22 in increasing the survival time of tumor-bearing mice and that the temperature of 60 °C for extraction was more efficient than 22 °C or 100 °C for the first two strains. Groups of animals were then sacrificed following the treatments with the extract from Le 96/17 or Le 95/01 obtained at 60 °C and evaluated on the NK activity, the lymphoproliferative responsiveness of spleen cells to concanavalin A, and the specific antibody production. NK activity and proliferative response were not affected by the treatments, whereas the antitumor antibody response was enhanced by Le 95/01. Further analysis demonstrated that the extracts in vitro inhibited the NK activity and the proliferative responsiveness to concanavalin A.