International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

For Online Access

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2004, Volume6

Issue 1

98 pages

DOI: 10.1615/IntJMedMushr.v6.i1

Issue price - $128.00

Yuexin Lin
The College of Bioengineering, Fujian Normal University, Fuzhou, R.P. China

Ping Lai
The College of Bioengineering, Fujian Normal University, Fuzhou, R.P. China

Yanyan Huang
The College of Bioengineering, Fujian Normal University, Fuzhou, R.P. China

Hualing Xie
The College of Bioengineering, Fujian Normal University, Fuzhou, R.P. China

Two main fractions of polysaccharide SL-I and SL-II-b were purified from submerged cultured mycelium of culinary—medicinal mushroom Lentinus strigellus using hot water extraction, ethanol precipitation, DEAE-cellulose chromatography, and Sephadex G-100 filtration. Polysaccharide analysis showed that SL-I is a protein-free polysaccharide with 1.15 × 10⁴ Da molecular weight and +63.33° optical rotation, while SL-II-b is a protein-conjugated polysaccharide with 3.88 × 10³ Da molecular weight and +73.67° optical rotation. The sugar constituents of polysaccharide analysis by GC showed that both fractions are heteropolysaccharides. SL-I consists of six monosaccharides, and SL-II-b consists of three monosaccharides. IR and ¹H-NMR, ¹³C-NMR spectra further indicated that LS-I and LS-II-b are heteropolysaccharides with a(1®2),(1®4) glucosidic bonds (LS-I), and a(1®2),(1®3),(1®4) glucosidic bonds (LS-II-b). The immune-competent examination showed that both polysaccharides could increase the spleen index, carbon clearance index, phagocytic index, and white blood cell levels of CY-treated mice.

DOI: 10.1615/IntJMedMushr.v6.i1.50 Download article, 8 pages

Article price - $35.00

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