International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

For Online Access

Best Paper Award Selection - Editorial Board Site

2005, Volume7

Issue 4

102 pages

DOI: 10.1615/IntJMedMushr.v7.i4

Issue price - $128.00

Ken-ichi Ishibashi
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan

Chikaku Dogasaki
Faculty of Environmental Health Sciences and School of Veterinary, Azabu University, Sagamihara, Kanagawa, Japan

Tsunenori Iriki
Faculty of Environmental Health Sciences and School of Veterinary, Azabu University, Sagamihara, Kanagawa, Japan

Masuro Motoi
Toei Pharmaceutical Co. Ltd., 2-5-3 Iguchi, Mitaka, Tokyo 181-0011, Japan

Yu-ichiro Kurone
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan

Noriko N. Miura
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan

Yoshiyuki Adachi
Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan

Naohito Ohno
Tokyo University Pharmaceutical and Life Sciences, School of Pharmacy, Laboratory Immunopharmacological Microbiological Production, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan

We recently detected anti-β-glucan (BG) antibody, which was highly reactive and specific to fungal β-1,3- and β-1,6-BG in sera from human volunteers and DBA/1 and DBA/2 mice. In this study, we examined whether the anti-BG antibody exists in bovine serum and compared the reactivity to various BG fractions from medicinal and edible mushrooms. In experiments using polysaccharide-coated ELISA plates, normal bovine serum showed higher levels of reactivity to the hot water extract of Agaricus brasiliensis, AgHWE. The relative activity is comparable to Candida solubilized cell wall BG, CSBG. On the other hand, the sera weakly reacted to grifolan (GRN) from Grifola frondosa and sonifilan (SPG) from Schizophyllum commune. Furthermore, in a competive ELISA, this antibody cross-reacted to the β-1,6-glucan segment of CSBG and AgHWE. Anti-BG antibody plays a role in the recognition of BG and induces biological activity in collaboration with other recognition molecules, such as the β-glucan receptor. These facts strongly suggest that cows show β-glucan-specific immune response, and β-glucan from mushrooms are recognized and induce immunopharmacological activity in cows.

DOI: 10.1615/IntJMedMushr.v7.i4.10 Download article, 513-524 pages

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