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International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

Institutional price:	$538.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2005, Volume7

102 pages

DOI: 10.1615/IntJMedMushr.v7.i4

Issue price - $128.00

Molecular Identification of Some Medicinally Important Aphyllophorales Mushrooms Based on ITS rDNA Sequences and RAPD Data

S. K. Singh
National Research Centre for Mushroom (ICAR), Chambaghat, Solan, HP, India

R. D. Rai
National Research Centre for Mushroom (ICAR), Chambaghat, Solan, HP, India

Shwet Kamal
National Research Centre for Mushroom (ICAR), Chambaghat, Solan, HP, India

ABSTRACT

Analysis of genetic relationship of important Aphyllophorales germplasm accessions of the National Research Centre for Mushroom, Solan, India, was conducted using RAPD and ITS rDNA sequences. RAPD profiles exhibited significant variations among Trametes versicolor, Tyromyces chioneus, and Ganoderma species. The resulting RAPD-based phylogram grouped Ganoderma spp. accessions into four clades. Phylogeny inferred from ITS rDNA sequence distinguished Trametes versicolor, Tyromyces chioneus, and Ganoderma spp. at the generic level due to different lengths and variations of the 5.8S rDNA conserved region. At the intrageneric level, Ganoderma accessions were divided into two distinct groups based on nucleotide substitution in ITS-1 and ITS-2 regions. It is presumed that some of the Ganoderma sp. samples might be G. lucidum. Single nucleotide substitutions turned out to be more useful for genetic cataloguing of isolates within a species than sequence alignment using NCBI databse.