International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

For Online Access

Best Paper Award Selection - Editorial Board Site

2001, Volume3

Issue 1

76 pages

Issue price - $128.00

Sergey V. Reshetnikov
N. G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, 2 Tereshchenkivskaya Str., Kiev 252001, Ukraine; and Med Myco Ltd., Institute of Evolution, University of Haifa, Mt. Carmel, Haifa 31905, Israel

Solomon P. Wasser
International Centre for Cryptogamic Plants and Fungi, Institute of Evolution, University of Haifa, Mt. Carmel, Haifa, Israel; and N.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kiev, Ukraine

Ina Duckman
Med Myco Ltd., Institute of Evolution, University of Haifa, Mt. Carmel, Haifa 31905, Israel

Katherina Tsukor
Med Myco Ltd., Institute of Evolution, University of Haifa, Mt. Carmel, Haifa 31905, Israel

Different yeast-like budding haploid strains of the yellow brain mushroom Tremella mesenterica (Retz.: Fr.) were obtained using the monobasidiosporous culture method from basidioma specimens collected in Israel. Nutritional requirements for biomass growth and extracellular polysaccharide production were investigated. A two-stage technology for acidic glucuronoxylomannan production was developed. On a first stage inoculum culture medium was balanced for biomass accumulation, and maximal polysaccharide production was obtained on a fermentation medium under conditions of nitrogen limitation. A crude product obtained by alcohol precipitation of culture broth contains 40% of acidic glucuronoxylomannan, 5% of neutral glucuronoxylomannan, 30% of cell biomass, and also includes low molecular weight substances, free amino acids, and mineral elements.

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