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International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

Institutional price:	$538.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2007, Volume9

98 pages

DOI: 10.1615/IntJMedMushr.v9.i2

Issue price - $149.00

Immunomodulatory and Antitumor Activities of Water-Soluble Proteoglycan Isolated from the Fruiting Bodies of Culinary-Medicinal Oyster Mushroom Pleurotus ostreatus (Jacq.: Fr.) P. Kumm. (Agaricomycetideae)

Saurabh Shah
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India

Dipanjan Ghosh
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India

Sanjaya Kumar Mallick
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India

Itisam Sarangi
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India

Sujit Kumar Bhutia
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India

Indranil Banerje
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India

Swatilekha Maiti
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India

Tapas K. Maiti
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India

ABSTRACT

Pleurotus ostreatus, also known as oyster mushroom, is studied for its immunomodulatory and anticancer properties. The present study aimed to find a bioactive proteoglycan from a fruiting body of P. ostreatus that could be used as a biological response modifier for immunomodulatory and anticancer effects with the fewest side effects. A water-soluble fraction of alcohol-precipitated fruiting body extract was purified through anion and cation exchange chromatography, followed by gel filtration (Sephadex G-100) chromatography. The purified proteoglycan fraction was represented by the polysaccharide-to-protein ratio of 3.46. The monosaccharide composition analysis of the proteoglycan showed the presence of galactose, mannose, and glucose in the ratio of 12:11:10. The fraction was examined for its immunomodulatory and anticancer effects in a mouse Sarcoma-180 model. The purified proteoglycan stimulated the growth of thymocytes and splenocytes and induced interleukin-4 and interferon gamma in vitro. It also activated macrophages to produce nitric oxide, super oxide anion, and hydrogen peroxide. The growth inhibition of Sarcoma-180 cells both in vivo and in vitro by this proteoglycan fraction was significant and arrested cell cycle in G₀/G₁ phase.