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International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

Institutional price:	$538.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2005, Volume7

344 pages

DOI: 10.1615/IntJMedMushr.v7.i12

Issue price - $256.00

Lectin from Tricholoma mongolicum S. Imai (Agaricomycetideae) Mycelia Stimulates Gene Expression of Immunomodulating Cytokines in Mouse Peritoneal Macrophages and Splenocytes

Fang Liu
Department of Microbiology, College of Life Science, Nankai University, Tianjin, China

Tzi Bun Ng
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

Hexiang Wang
Department of Microbiology, China Agricultural University, Beijing, China

Ming Chiu Fung
Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong, China

Vincent Eng Choo Ooi
Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories Hong Kong, China

ABSTRACT

The effects of lectin from the edible mushroom Tricholoma mongolicum on cytokine gene expression were studied. RNA was extracted from splenocytes and peritoneal exudate cells 24 hours after the last of a series of 10 daily intraperitoneal injections (10 μg/mouse/injection), and reverse transcription/polymerase chain reaction was employed in the analysis of cytokine gene expression. In peritoneal exudate cells, the level of gene expression of inducible nitric oxide synthetase, interleukin (IL)-1β, and transforming growth factor-β was upregulated by the lectin. In spleen cells, the level of gene expression of inducible IL-1β was upregulated.