ITEMS

modern scholarly publishers in the finest tradition

Journals

	Search

International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

Institutional price:	$538.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2003, Volume5

130 pages

DOI: 10.1615/InterJMedicMush.v5.i2

Issue price - $128.00

Purification of Caffeic Acid as an Antioxidant from Submerged Culture Mycelia of Phellinus linteus (Berk. et Curt.) Teng (Aphyllophoromycetideae)

Tomoyuki Nakamura
Applied Fungi Institute, IBI Corporation, 7841 Anayama-cyo, Nirasaki-shi, Yamanashi 407-0263; and Faculty of Engineering, Yamanashi University, 4-3-11 Takeda, Kofu-shi, Yamanashi 400-8511, Japan

Yukihito Akiyama
Applied Fungi Institute, IBI Corporation, 7841 Anayama-cyo, Nirasaki-shi, Yamanashi 407-0263, Japan

Seiichi Matsugo
Faculty of Engineering, Yamanashi University, 4-3-11 Takeda, Kofu-shi, Yamanashi 400-8511, Japan

Yasuyuki Uzuka
Faculty of Engineering, Yamanashi University, 4-3-11 Takeda, Kofu-shi, Yamanashi 400-8511, Japan

Keiji Shibata
Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan

Hirokazu Kawagishi
Department of Applied Biological Chemistry, Faculty of Agriculture, Graduate School of Science and Technology, Shizuoka University, Shizuoka, Japan

ABSTRACT

Phellinus linteus mycelia have many pharmacological effects although their pharmacological efficacy principles have not been known before. In the course of screening for biological activity of the extracts of Ph. Linteus mycelia, we found strong antioxidative activity in the extract. Therefore, we tried to isolate the active principle(s) from the extract. The isolation of the active compound was guided by superoxide anion radical scavenging activity. As a result, caffeic acid was isolated as an active compound. The IC₅₀ of the compound was 3.05 μg/mL (16.9 μM).