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International Journal of Medicinal Mushrooms

 

ISSN for PRINT: 1521-9437

Institutional price:

$538.00

Issues per year:

4

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Best Paper Award Selection - Editorial Board Site

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2002, Volume4

Issue 2

  106 pages  

   

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Issue price - $128.00  

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  • Characterization of Somatic Hybrids with Compatible and Incompatible Species by Protoplast Fusion in Genera Pleurotus (Fr.) P. Karst. and Ganoderma P. Karst. by RAPD-PCR Analysis
  • Young Bok Yoo
    Applied Microbiology Division, National Institute of Agricultural Science and Technology, RDA, Suwon 441-707, South Korea

    Kang Hyo Lee
    Applied Microbiology Division, National Institute of Agricultural Science and Technology, RDA, Suweon 441-707, South Korea

    Beom Gi Kim
    Applied Microbiology Division, National Institute of Agricultural Science and Technology, RDA, Suwon 441-707, South Korea


    ABSTRACT

    A comparison of somatic hybrids with compatible and incompatible strains was made using random amplified polymorphic DNA (RAPD) analysis. The heterokaryons between compatible species, Pleurotus ostreatus and P. florida, showed the same level of variability and contained both parental RAPD bands. In contrast, most of the synkaryons between incompatible species such as P. cornucopiae + P. florida, P. ostreatus + Ganoderma applanatum, and P. florida + Ganoderma lucidum showed similarilarity to those of either parental bands or nonparental RAPD bands. Synkaryons can be classified into microgenome insertion type and macrogenome insertion type.

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