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International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

Institutional price:	$538.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2004, Volume6

104 pages

DOI: 10.1615/IntJMedMushr.v6.i2

Issue price - $128.00

Extracellular Polysaccharide Production by Culinary-Medicinal Shiitake Mushroom Lentinus edodes (Berk.) Singer and Pleurotus (Fr.) P. Karst. Species Depending on Carbon and Nitrogen Source

Vladimir Elisashvili
Durmishidze Institute of Biochemistry and Biotechnology, Academy of Science of Georgia, 10 km Agmashenebeli kheivani, 0159 Tbilisi, Georgia

Solomon P. Wasser
International Centre for Cryptogamic Plants and Fungi, Institute of Evolution, University of Haifa, Mt. Carmel, Haifa, Israel; and N.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kiev, Ukraine

Kok- Kheng Tan
MycoBiotech Pte Ltd 12 Science Park Drive # 04-01 The Mendel- Singapore Science Park 1 118 225, Singapore

David Chichua
Institute of Biochemistry and Biotechnology, Academy of Science of Georgia, Agmashenebeli Alley 10 km, 380059 Tbilisi, Georgia

Eva Kachlishvili
Durmishidze Institute of Biochemistry and Biotechnology, Academy of Science of Georgia, 10 km Agmashenebeli kheivani, 0159 Tbilisi, Georgia

ABSTRACT

The effects of different carbon and nitrogen sources on culinary—medicinal shiitake mushroom Lentinus edodes and Pleurotus species growth and polysaccharide production in submerged cultivation has been investigated. Maximal biomass and extracellular polysaccharide (EPS) production was achieved when sodium gluconate or glucose was used as the only carbon source and peptone or corn steep liquor as the nitrogen source. The cultivation process in shake flasks could be successfully reproduced in a laboratory bioreactor.