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International Journal of Medicinal Mushrooms

 

ISSN for PRINT: 1521-9437

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$538.00

Issues per year:

4

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2004, Volume6

Issue 2

  104 pages  

DOI: 10.1615/IntJMedMushr.v6.i2   

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  • Analysis of Quality and Techniques for Hybridization of Medicinal Fungus Cordyceps sinensis (Berk.)Sacc. (Ascomycetes)
  • John C. Holliday
    Aloha Medicinals Inc., 1211 Fair Avenue Santa Cruz, CA 95060 USA

    Phillip Cleaver
    Aloha Medicinals Inc., Haiku, Hawaii, USA

    Megan Loomis-Powers
    Aloha Medicinals Inc., Haiku, Hawaii, USA

    Dinesh Patel
    Integrated Biomolecule Corporation, Tucson, Arizona, USA


    ABSTRACT

    In the course of our research with cultivated medicinal fungus Cordyceps sinensis, we have noted a greater diversity of compounds from different strains of this single species than in almost any other organism we have analyzed. Because of this great difference in chemical composition, a wide range of quality is found in cultivated C. sinensis. This article details the unique methods used to develop hybridized strains of C. sinensis using rattlesnake venom to trigger somatic fusion of dissimilar mycelial strains.This results in the reproducible production of cultivated C. sinensis containing quantities of recognized bioactive compounds equal to or greater than the quantities found in wild collected strains.These techniques and methods offer great promise in allowing cultivators of C. sinensis and other Cordyceps species to take their artificially cultivated products to a higher and more consistent level of quality and provide protocols for analysis of C. sinensis quality using HPLC and GC techniques.

    DOI: 10.1615/IntJMedMushr.v6.i2.60

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