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International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

Institutional price:	$538.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2008, Volume10

104 pages

DOI: 10.1615/IntJMedMushr.v10.i1

Issue price - $161.00

Antitumor Properties of Submerged Cultivated Biomass and Extracts of Medicinal Mushrooms of Genus Hypsizygus Singer (Agaricomycetideae)

Larissa M. Krasnopolskaya
Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, 11 B, Pirogovskaya Str., Moscow 119867, Russia

Maria I. Leontieva
Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, Moscow, Russia

Anastasia V. Avtonomova
Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, B. Pirigovskaya St., 11,119021 Moscow, Russia

Elena B. Isakova
Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, B. Pirigovskaya St., 11,119021 Moscow, Russia

Igor V. Belitsky
Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, 11 B, Pirogovskaya Str., Moscow 119867, Russia

Anatoliy I. Usov
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 47,117913 Moscow, Russia

Vladimir M. Bukhman
Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, B. Pirigovskaya St., 11,119021 Moscow, Russia

ABSTRACT

The present article describes the development of media for high-yield cultivation of medicinal mushrooms Hypsizygus marmoreus and H. ulmarius and the results of in vivo investigations into the antitumor activity of the water extracts of the mycelia of both species and of the total polysaccharide fraction of H. ulmarius. The media were optimized using statistical experimental design methodologies, including full factorial design and steepest ascent. The mycelia yield of H. ulmarius amounted to 32-33 g/L of air-dried biomass on the 3rd to 4th day of cultivation. The corresponding figures for two strains of H. marmoreus, Hm-1 and Hm-2, were 26 and 17 g/L, respectively, on the 7th day of cultivation. The water content of air-dried biomass did not exceed 5%. The antitumor activity was studied in vivo on adult male hybrid mice (C57Bl/6 × DBA/2)F1 with transplantable tumor T-cell lymphoma P388. The inhibition ratio (IR) of tumor growth reached 80% for the water extract of H. ulmarius mycelium and 38% and 59%, respectively, for the two strains of H. marmoreus. The water-soluble polysaccharide fraction of H. ulmarius mycelium contained glucose, galactose, mannose, rhamnose, arabinose, and xylose in the relative amounts of 23.5, 9.5, 7, 2, 1.5, and 1, respectively; its antitumor activity was tested in 2 doses, 2 and 20 mg/kg/day. The 2 mg/ kg/day dose was more effective than either the 20 mg/kg/day dose or the mycelium water extract. The results prove H. ulmarius to be a promising source of antitumor polysaccharides.