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International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

Institutional price:	$538.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2008, Volume10

104 pages

DOI: 10.1615/IntJMedMushr.v10.i1

Issue price - $161.00

Effect of the Carbon Source and Inoculum Preparation Method on Laccase and Manganese Peroxidase Production in Submerged Cultivation by the Medicinal Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae)

George G. Songulashvili
Institute of Evolution and Department of Evolutionary and Environmental Biology, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, Haifa, Israel; and Durmishidze Institute of Biochemistry and Biotechnology, 0159 Tbilisi, Georgia

Vladimir Elisashvili
Durmishidze Institute of Biochemistry and Biotechnology, Academy of Science of Georgia, 10 km Agmashenebeli kheivani, 0159 Tbilisi, Georgia

Solomon P. Wasser
International Centre for Cryptogamic Plants and Fungi, Institute of Evolution, University of Haifa, Mt. Carmel, Haifa, Israel; and N.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kiev, Ukraine

Yitzhak Hadar
Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University, P. 0. Box 12, Rehovot 76100, Israel

Eviatar Nevo
Institute of Evolution, Haifa University, Mount Carmel, 31905 Haifa, Israel

ABSTRACT

The genus Ganoderma includes medicinal species that belong to the group of white rot fungi due to their ability to produce extracellular ligninolytic enzymes: laccase and manganese peroxidase (MnP). The aim of this study was to evaluate the significance of various carbon and inoculum preparation methods for oxidative enzyme production by the medicinal mushroom G. lucidum. Mushroom growth, as well as the production of laccase and MnP, significantly depends on the inoculum age and cultivation method. Synthetic medium experiments have shown that xylose ensured the highest laccase activity of G. lucidum 447 accumulating 116 U L⁻¹ after 12 days of submerged mushroom cultivation. In all lignocellulosic substrate experiment variants, laccase activity reached maximum after 8 days of mushroom cultivation. Analogical regularities were detected when the MnP activity was measured in submerged fermentation of ethanol production residue by G. lucidum 447. A more interesting finding is the fact that mycelium grown on solid agar medium and used as inoculum not only provided a 3-fold increase in laccase yield in submerged fermentation of both lignocellulosic substrates but also stimulated MnP accumulation by G. lucidum 447.