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International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

Institutional price:	$538.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2008, Volume10

104 pages

DOI: 10.1615/IntJMedMushr.v10.i1

Issue price - $161.00

The Role of Lectin-Carbohydrate Biospecific Interactions between Medicinal Basidiomycetes Mushroom Grifola frondosa (Dicks.: Fr.) S. F. Gray and Azospirillum brasilense During Their Co-cultivation

Lada V. Stepanova
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov 410049, Russia

Andrei V. Schelud'ko
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov 410049, Russia

Elena I. Katsy
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov 410049, Russia

Elena G. Ponomareva
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov 410049, Russia

Valentina E. Nikitina
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov 410049, Russia

ABSTRACT

We studied the role of lectin-carbohydrate recognition as a biospecific interaction during co-cultivation of the culinary-medicinal Basidiomycetes species Grifola frondosa and the diazotrophic bacterium Azospirillum brasilense Sp245 on an agar-supplemented medium. We showed that A. brasilense Sp245 and its Omegon-Km mutants, KM018 and KM139, defective in lipopolysaccharide (LPS) structure, whose LPSs contain O-specific polysaccharide (acidic OPS1, linear D-rhamnan), a hapten for the fungal lectin, are recognized by the fungus with the least antagonism, as distinct from mutants KM252 and KM348, which have neutral OPS2 and are inhibitory to the growth of the G. frondosa 0917 mycelium. Studying the influence of a pure lectin preparation on the motilities of these strains in a liquid medium, with the exception of the immotile KM018, revealed a decrease in the swimming speeds of Sp245 and KM139. The motilities of KM252 and KM348 were almost unaffected by the lectin. The obtained results attest to the involvement of biospecific lectin-carbohydrate interactions in the primary contact between the G. frondosa 0917 mycelium and the bacterium A. brasilense during co-cultivation. It is most probable that, in this case, the lectin acts as an attractant-like factor on the bacteria, thus ensuring the recognizing and protective functions of the fungus.