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International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

Institutional price:	$538.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2003, Volume5

102 pages

DOI: 10.1615/InterJMedicMush.v5.i1

Issue price - $128.00

Production of Polysaccharide by Culinary-Medicinal Mushroom Agaricus brasiliensis S. Wasser et al. LPB 03 (Agaricomycetideae) in Submerged Fermentation and Its Antitumor Effect

Leifa Fan
Laboratorio de Processos Biotecnologicos, Departamento de Engenharia Quimica, Universidade Federal do Parana. Centro Politecnico, Curitiba PR, CEP: 81531-990, Brazil

Andrea Thomaz Soccol
Laboratorio de Processos Biotecnologicos, Departamento de Engenharia Quimica, Universidade Federal do Parana. Centro Politecnico, Curitiba PR, CEP: 81531-990, Brazil

Ashok Pandey
Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum-695 019, India; and 3Instituto de Tecnologia do Parana, TECPAR, CIC, Curitiba PR, CEP: 81350-010, Brazil

Sandro Germano
Laboratorio de Processos Biotecnologicos, Departamento de Engenharia Quimica, Universidade Federal do Parana. Centro Politecnico, Curitiba PR, CEP: 81531-990, Brazil

Renato Rau
Instituto de Tecnologia do Parana, TECPAR, CIC, Curitiba PR, CEP: 81350-010, Brazil

Aurora L. Pedroso
Instituto de Tecnologia do Parana, TECPAR, CIC, Curitiba PR, CEP: 81350-010, Brazil

Carlos Ricardo Soccol
Laboratorio de Processos Biotecnologicos, Departamento de Engenharia Quimica, Universidade Federal do Parana. Centro Politecnico, Curitiba PR, CEP: 81531-990, Brazil

ABSTRACT

Studies were performed on the production of extracellular polysaccharide in submerged culture of Agaricus brasiliensis Wasser et al. and the effects of different carbon and nitrogen sources individually and in combinations on production. The polysaccharide produced was evaluated for its antitumor action against Sarcoma 180. Glucose and yeast extract gave the best combination of carbon and nitrogen sources for the polysaccharide production. The biomass yield and polysaccharide produced were 222.3 and 10.867 mg/50 mL (dry wt), respectively, at the concentration of 20 g/L glucose and 2 g/L yeast extract with the medium containing MgSO₄Ч7H₂O (0.1%) and K₂HPO₄Ч3H₂O (0.1%) at pH 6.0 and 32°C after the 9 days' static cultivation. The extracellular polysaccharide showed strong antitumor activity against Sarcoma 180. Complete regression ratio of 50% and suppression ratio percentage of 72.19% were observed.