International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

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2002, Volume4

Issue 4

112 pages

Issue price - $128.00

Lin Yuexin
Bioengineering College, Fujian Teachers University, Fuzhou 350007, People's Republic of China

Ye Zhuqiu
Bioengineering College, Fujian Teachers University, Fuzhou 350007, People's Republic of China

Huang Yanyan
Bioengineering College, Fujian Teachers University, Fuzhou 350007, People's Republic of China

Xie Hualing
Bioengineering College, Fujian Teachers University, Fuzhou 350007, People's Republic of China

Three groups of water-soluble polysaccharides, AB-I, AB-II-b, and AB-III-b, were obtained from Agaricus blazei fruit body by hot-water extraction, ethanol precipitation, DEAE-cellulose chroniatography, and Sephadex G-100 gel filtration. HPLC examination showed that they were purified into pure preparations. The molecular weight, monosaccharide constituent, and glucosidic bond of the polysaccharides were tested by employing Sepharose CL-4B, GC, and IR. The results were as follows: molecular weight: AB-I, 21 ґ 10⁴, AB-II-b, 34 ґ 106, AB-III-b, 6 ґ 104; monosaccharide composition: AB-I, Gal:Man:Glc (1.43:2.02:5.57), AB-II-b, Rha:Gal:Man:Glc (1.00:1.26:2.75:4.26), AB-III-b, Rha:Xyl:Gal:Man:Glc (1.49:0.37:0.84:1.86:4.05). IR examination showed that the glucosidic bond of the three polysaccharides were b-pyran type. The polysaccharides contained a protein constituent.

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