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International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

Institutional price:	$538.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2008, Volume10

121 pages

Issue price - $161.00

Purification and Particular Characterization of Laccase from the Ling Zhi or Reishi Medicinal Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. 447 (Aphyllophoromycetideae)

George G. Songulashvili
Institute of Evolution, Department of Evolutionary and Environmental Biology, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, Haifa, 31905 Israel; and Durmishidze Institute of Biochemistry and Biotechnology,0159 Tbilisi, Georgia

Vladimir Elisashvili
Durmishidze Institute of Biochemistry and Biotechnology, Academy of Science of Georgia, 10 km Agmashenebeli kheivani, 0159 Tbilisi, Georgia

Solomon P. Wasser
International Centre for Cryptogamic Plants and Fungi, Institute of Evolution, University of Haifa, Mt. Carmel, Haifa, Israel; and N.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kiev, Ukraine

Eviatar Nevo
Institute of Evolution, and Department of Evolutionary and Environmental Biology, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, Haifa, 31905 Israel

Yitzhak Hadar
Department of Plant Pathology and Microbiology, Faculty of Agricultural and Environmental Quality Sciences, the Hebrew University, Rehovot 76100, Israel

ABSTRACT

The Ganoderma lucidum strain 447 was cultivated in a 10-L fermentor. We used the ethanol-production residue (by wheat) (REP) as the growth substrate and Cu as an inductor. Ganoderma lucidum was grown for 8 days before laccase reached the highest yield (188,600 U L⁻¹). The purification enzyme appeared as two-laccase isozyme bands on SDS-PAGE. The molecular masses were 43 and 56 kDa by SDS-PAGE. The pH optimum for 2,2'-azino-bis-[3-ethyltiazoline-6-sulfonate] (ABTS) oxidation was 3 in citric/acetic buffers. In this study, the optimum temperature for laccase activity was determined to be 30°C. The kinetic of laccase was experimented on using 12 phenolic substrates. The lowest Km values (0.0048 and 0.005 mM) were found for syringaldazine and ABTS, respectively.