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International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

Institutional price:	$538.00
Issues per year:	4

Best Paper Award Selection - Editorial Board Site

2006, Volume8

108 pages

DOI: 10.1615/IntJMedMushr.v8.i4

Issue price - $138.00

Effect of Culture Conditions on the Chemical Composition and Biological Activities of Agaricus brasiliensis S.Wasser et al. (Agaricomycetideae)

Satoshi Hashimoto
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan

Akiko M. Akanuma
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan

Masuro Motoi
Toei Pharmaceutical Co. Ltd., 2-5-3 Iguchi, Mitaka, Tokyo 181-0011, Japan

Nobuhiro Imai
Guinishi Alimentary Production Export-and-Import Limited Company, Estrada Dos Fernandes 2563, Suzano, Sao Paulo, Brazil

Carlos A. Rodrignes
Guinishi Alimentary Production Export-and-Import Limited Company, Estrada Dos Fernandes 2563, Suzano, Sao Paulo, Brazil

Sachiko Nameda
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan

Noriko N. Miura
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan

Yoshiyuki Adachi
Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan

Naohito Ohno
Tokyo University Pharmaceutical and Life Sciences, School of Pharmacy, Laboratory Immunopharmacological Microbiological Production, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan

ABSTRACT

Agaricus brasiliensis is included in many kinds of health foods. Both the fruit body and mycelium, cultured in various countries from various strains and using various production processes, are supplied. In the present study, we planned to compare the chemical properties and biological activities of A. brasiliensis cultured under different conditions, i.e., indoors in a house (IC) and outside on a field (OC), using the same strain and the same production process. Polysaccharide fractions prepared from IC and OC had similar structural features and biological activity, as assessed by component sugar analysis, nuclear magnetic resonance, and antitumor activity in vivo. In contrast, the protein fractions were qualitatively different, i.e., specific protein spots were detected by two-dimensional polyacrylamide gel electrophoresis, and the enzyme activities, especially laccase, polyphenoloxidase, peroxidase, and beta-glucanase activities, were different. These facts strongly suggested that the culture conditions of medicinal mushrooms are keys for their pharmacological activity assessment.