Annals of the Assembly for International Heat Transfer Conference 13

ISBN 1-56700-225-0 / CD 1-56700-226-9

Year 2006

Atsuki Komiya
Institute of Fluid Science, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan

Shigenao Maruyama
CIRPS—Institute of Fluid Science, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai, 980-8577, Japan

S. Moriya
Tohoku University, Sendai, Japan

This paper deals with a novel approach for developing of measurement system for mass diffusion coefficients of micro quantity proteins by using phase shifting interferometer. The measurement system developed in this study could solve several measurement difficulties and accomplish quick and precise measurement of mass diffusion coefficient. For the observation of small transient diffusion field, Mach-Zehnder type phase shifting interferometer and specially designed shearing cell were utilized. The present small shearing cell designed requires only 10 micro liter solutions to form the transient diffusion field. As a target protein, lysozyme extracted from albumen was used. For the avoidance of protein unfolding, the lysozyme was dissolved in buffer solution that is sodium acetate (CH₃COONa) with pH 4.5. The experimental results indicated that the concentration profile in a diffusion field could be detected clearly even though the field of view is smaller than 2.0mm square. The mass diffusion coefficient was derived by a novel analytical method proposed by authors. This method can derive mass diffusion coefficient as a function of concentration from one measurement datum. From the experimental data, the dependency of mass diffusion coefficient on environmental conditions was discussed.

MST-10 pages DOI: 10.1615/IHTC13.p10.100 Download article

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