Experimental investigation was conducted on the phenomenon of cooled air injection from a thermoacoustic refrigerator. The location of the stack, the diameter and the position of the air outlet hole, and electrical thermal input were varied in the experiments. Axial pressure distribution in the resonance tube was measured by a movable pressure transducer along the tube axis. Refrigeration capacity was measured with electrical thermal input. Air, a working gas in the tube in this experiment, was cooled by thermoacoustic phenomena and was naturally flown out through the outlet hole near the pressure antinode. Ambient air was flown in through the inlet hole near the pressure node. The air streams naturally flowed in and out through the holes without any one-way valve.